南京林業(yè)大學(xué)學(xué)報(bào)（自然科學(xué)版）
    JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
    2002 Vol.26 No.3 P.85-90

    植物抗病信號傳導(dǎo)途徑及其相互作用
    Signaling Pathways and Interplay in Plant Disease Resistance
    陳英　黃敏仁　諸葛強(qiáng)　王明庥　

    摘　要：植物與病原物長期的互作過程中產(chǎn)生了一系列的防衛(wèi)反應(yīng),其中系統(tǒng)獲得性抗性(Systemic Acquired Resistance,SAR)和誘導(dǎo)性系統(tǒng)抗性(Induced Systemic Resistance,ISR)是植物抗病信號傳導(dǎo)途徑中的兩種重要形式.它們分別由植物內(nèi)源信號分子SA和JA/Et作介導(dǎo),兩種信號的傳導(dǎo)途徑之間既相互獨(dú)立又相互聯(lián)系,協(xié)同作用,從而使植物作出對自身傷害最小卻又最為有效的防衛(wèi)反應(yīng).筆者就SA-依賴性信號傳導(dǎo)途徑和SA-非依賴性信號傳導(dǎo)途徑的分子生物學(xué)研究進(jìn)展,以及兩種信號傳導(dǎo)途徑間的相互作用等方面進(jìn)行了綜述.

    關(guān)鍵詞：系統(tǒng)獲得性抗性;誘導(dǎo)性系統(tǒng)抗性;信號傳導(dǎo)
    分類號：S722 　文獻(xiàn)標(biāo)識碼：A
    文章編號：1000-2006(2002)03-0085-06
    基金項(xiàng)目：江蘇省高新技術(shù)資助項(xiàng)目(BG2001312)
    作者簡介：陳英(1973-),女,四川宜賓人,南京林業(yè)大學(xué)林木育種專業(yè)博士生.
    作者單位：陳英（南京林業(yè)大學(xué)林木遺傳和基因工程重點(diǎn)實(shí)驗(yàn)室,江蘇,南京,210037）　
　　　　　    黃敏仁（南京林業(yè)大學(xué)林木遺傳和基因工程重點(diǎn)實(shí)驗(yàn)室,江蘇,南京,210037）　
　　　　　    諸葛強(qiáng)（南京林業(yè)大學(xué)林木遺傳和基因工程重點(diǎn)實(shí)驗(yàn)室,江蘇,南京,210037）　
　　　　　    王明庥（南京林業(yè)大學(xué)林木遺傳和基因工程重點(diǎn)實(shí)驗(yàn)室,江蘇,南京,210037）　

    參考文獻(xiàn)：
    ［1］Goodman R N,Novacky A J.The hypersensitive reaction in plants to Pathogenes[M].APS Press,1994.
    ［2］Ross A F.Systemic acquired induced by localized virus infections in plants[J].Virology 14,1991(b):340-358.
    ［3］Pieterse C M J,VanWees SCM,Pelt JAV,et al.A novel signaling pathway controlling induced systemic resistance in Arabidopsis[J].Plant Cell,1998,10:1?571-1?580.
    ［4］Bart J F,Jane E P.Interplay of signaling pathways in plant disease resistance[J].Trends in Genetics,2000,10:449-455.
    ［5］Morrissey J P,Osbourn A E.Fungal resistance to plant antibiotics as a mechanism of pathogenesis[J].Miorobiol Mol Biol Rev,1999,63:708-724.
    ［6］Malamy J,Carr J P,Klessig D F,et al.Salicylic acid:A likely endogenous signal in the resistance response of tobacco to viral infection[J].Science,1990,250:1?002-1?004.
    ［7］Mtraux J P,Singer H,Ryals J A,et al.Increase in salicylic acid at the onset of systemic acquried resistance in cucumber[J].Science,1990,250:1?004-1?006.
    ［8］Yalpani N,Silverman P,Wilson T M A,et al.Salicylic acid is a systemic signal and an inducer of pathogenesis-related protein in virus-infected tobacco[J].Plant Cell,1991,3:808-818.
    ［9］Vernooij B,Friedrich L,Reist R,et al.Salicylic acid is not the translocated signal responsible for inducing systemic acquired resistance is required in signal transduction[J].Plant Cell,1994,6:959-965.
    ［10］Chen Z,Silva H,Klessig D F.Involvement of reactive oxygen species in the induction of systemic acquired resistance by salicylic acid in plants[J].Science,1993,242:883-886.
    ［11］Chen Z X,Malamy J,Henning J,et al.Induction,modification,and transduction of the salicylic acid signal in plant defense response[J].Proc Natl Acad Sci USA,1995,92:4?134-4?137.
    ［12］余迪求,岑川,楊明蘭,等.水楊酸誘導(dǎo)煙草培養(yǎng)細(xì)胞的脂質(zhì)過氧化和保護(hù)基因表達(dá)的研究[J].植物學(xué)報(bào),1999,41:977-983.
    ［13］Durner J,Klessig D F.Salicylic acid is a modulator of tobacco and mammalian catalases[J].J Biol Chem,1996,27:28?492-28?501.
    ［14］Hammond KKE,Jones JG.Resistance gene-dependent plant defense response[J].Plant Cell,1997,11:993-969.
    ［15］Bi YM,Kenton P,Mur L,et al.Hydrogen peroxide does not function downstream of salicylic acid in the induction of PR protein expression[J].Plant J,1995,8:235-245.
    ［16］León J,Lawton M A,Raskin I.Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco[J].Plant Physiol,1995,108:1?673-1?678.
    ［17］Neuenschwander U,Vernooij B,Friedrich L,et al.Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance?[J].Plant J,1995,8:227-233.
    ［18］Summermatter K,Sticher L,Métraux J P.Systemic response in Arabidopsis thaliana infected and challenged with Pseudomonas syringae pv.syringae[J].Plant Physiol,1995,108:1?379-1?385.
    ［19］Glazebrook J,Rogers E E,Ausubel F M.Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening[J].Genetics,1996,143:973-982.
    ［20］Zhou N.PAD4 functions upstream from salicylic acid to control defense response in Arabidopsis[J].Plant Cell,1998,10:1?021-1?030.
    ［21］Jirage D,Tootle T L,Reuber T L,et al.Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling[J].Proc Natl Acad Sci USA,1999,96:13?583-13?588.
    ［22］Falk A,Feys B J,Frost L,et al.EDS1,an essential component of R gene mediated disease resistance in Arabidopsis has homology to eukaryotic lipases[J].Proc Natl Acad Sci USA,1999,96:3?292-3?297.
    ［23］Nawrath C,Métraux J P.Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen incolation[J]. Plant Cell,1999,11:1?393-1?404.
    ［24］Reuber T,Plotikova J M,Dewdney J,et al.Correlation of defence gene induction defects with powdery mildew susceptibility in Arabidopsis enhanced disease susceptibility mutants[J].Plant J,1998,16:473-485.
    ［25］Roger E E.Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogen and alterations in PR-1 gene expression[J].Plant Cell,1997,9:305-316.
    ［26］Dempsey D M,Wobbe K,Klessig D F.Salicylic acid and disease resistance in plant[J].Crit Rev Plant Sci,1999,63:707-724.
    ［27］Cao H,Glazebrook J,Clark J D,et al.The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J].Cell,1997,88:57-63.
    ［28］Zhang Y L,Fan W H,Kinkema M,et al.Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J].Proc Natl Acad Sci USA,1999,96:6?523-6?528.
    ［29］Zhou J M,Trifa Y,Silva H,et al.NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid[J].Mol Plant Microbe Interact,2000,13:191-202.
    ［30］Despres C,Delong C,Glaze S,et al.The Arabidopsis NPR1/NIM1 protein enhanced the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors[J].Plant Cell,2000,12:279-290.
    ［31］Li X,Zhang Y L,Joseph D,et al.Identification and cloning of a negative regulator of systemic acquired resistance,SNI1,through a screen for suppressors of npr1-1[J].Cell,1999,98:329-339.
    ［32］Thomma B P H J,Schmidt S,Zheng X Y,et al.Requirement of function ethylene-insensitive 2 gene for efficient resistance of Arabidopsis to infection by Botrytis cinerea[J].Plant Physiol,1999,119:935-949.
    ［33］Penninckx I A M A,Eggermont K,Terras T R G,et al.Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid independent pathway[J].Plant Cell,1996,8:2?309-2?323.
    ［34］Penninckx I A M A,Thomma B P H J,Buchala A,et al.Concomitant activation of jasmonate and ethylene response pathway is required for induction of a plant defensin gene in Arabidopsis[J].Plant Cell,1998,10:2?103-2?113.
    ［35］Thomma B P H J,Eggermont K,Penninckx I A M A,et al.Separete jasmonate-dependent and salicylic acid dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens[J].Proc Natl Acad Sci USA,1998,95:15?107-15?111.
    ［36］Thomma B P H J,Nelissen L,Eggermont K,et al.Deficiency in phytoalexin production causes enhanced susceptibility of Arabidopsis thaliana to fungal Alternaria brassicicola[J].Plant J,1999,19:163-171.
    ［37］Zhou N,Tootle T L,Glazebriik J.Arabidopsis PAD3,a gene required for camalexin biosythesis,encodes a putative cytochrome P450 monooxygenase[J].Plant Cell,1999,11:2?419-2?428.
    ［38］Glazebrook J,Zook M,Mert F,et al.Phytoalexin-deficient mutants of Arabidopsis reveal that PAD4 encodes a regulatory factor and that four PAD genes contribute to downy mildew resistance[J].Genetics,1997,146:381-392.
    ［39］Lee M W,Qi M,Yang Y.A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death[J].Mol Plant Microbe Interact,2001,14:527-535.
    ［40］Penacortes H,Albrecht T,Prat S,et al.Aspirin prevents wounds-induced gene-expression in tomato leaves by blocking jasmonic acid biosysthesis[J].Planta,1993,191:123-128.
    ［41］Doares S H.Salicylic acid inhibits synthesis of protienase inhibitors in tomato leaves induced by systemin and jasmonic acid[J].Plant Physiol,1995,108:1?741-1?746.
    ［42］Reymond P,Farmer E E.Jasmonate and salicylate as global signals for defense gene expression[J].Curr Opin Plant Biol,1998,1:404-411.
    ［43］Bowling S A,Clarke J D,Liu Y D.et al.The cpr5 mutant of Arabidopsis express both NPR1-dependent and NPR1-independent resistance[J].Plant Cell,1997,9:1?573-1?584.
    ［44］Clarke J D,Liu Y D,Daniel F K,et al.Uncoupling PR gene expression from NPR1 and bacterial resistance:Characterization of the dominant Arabidopsis cpr6-1mutant[J].Plant Cell,1998,10:557-569.
    ［45］Shah J,Kachroo P,Glanzebrook J.The Arabidopsis ssi1 mutation restores pathogenesis-related gene expression in npr1 plant and renders defensin gene expression salicylic acid dependent[J].Plant Cell,1999,11:191-206.
    ［46］Richberg M H.Dead cells do tell tales[J].Curr Opin Plant Biol,1998,1:480-485.
    ［47］Clark J D,Volko S M,Ledford H,et al.Roles of salicylic acid,jasmonic acid,and ethylene in cpr-induced resistance in Arabidopsis[J].Plant Cell,2000,12:2?175-2?190.